Journal: bioRxiv

Article Title: Targeting Nitric Oxide Synthase 2 Reverses Learning Deficits in an Oligodendrocyte-Focused Model of Costello Syndrome

doi: 10.64898/2026.06.01.729333

Figure Lengend Snippet: A) Recombination strategy for tamoxifen (tmx; cyan)–mediated replacement of the HRas gene (grey, flanked by loxP sites) with HRasG12V (purple) in PlpCreERt2;FR-HRasG12V (pHRsG/+) mice. B) 2-month-old (2MO) mice treated with tmx are subjected to the complex wheel (CW) test, as well as histological and DTI-MRI analyses, 1-16 weeks later. C) Cell populations are analyzed in seven regions throughout the anterior-posterior (I-IV) and lateral “B” - central “C” axes of the corpus callosum (CC), unless otherwise disclosed. D) Immunostaining of coronal sections showing recombinant cells (GFP+, green arrowheads), OLs (GSTpi+, purple arrowheads), and GFP+GSTpi+ recombinant OLs (orange arrowheads) in the CC of WT and pHRsG/+ mice. E) Bar graph showing that the percentage (normalized to dapi) of recombinant cells in WT and pHRsG/+ mice is not significantly different (unpaired Student’s t test; males P= 0.14 and females P= 0.24). Genotype/sex color code and “n” per group in E, G : male WT (green) and pHRsG/+ (orange); female WT (black) and pHRsG/+ (red). F) Immunostaining picture indicating recombinant cells (green arrowheads), microglia (IBA1+, blue arrowheads), and OPCs (PDGFRa+, orange arrowheads) in the CC of WT and pHRsG/+ mice. G) The percentage of IBA+ cells in WT and pHRsG/+ mice (% of age/gender matched WTs) indicates significantly decreased # of microglia in male pHRsG/+ mice (unpaired Student’s t test; P= 0.047). Insets in D and F show the CC region in high magnification (dotted yellow square). Dapi was used to stain nuclei and normalize cell densities per arbitrary units (AU). Scale bar = 25μm. *P < 0.05.

Article Snippet: Floating sections were processed for immunodetection using antibodies for the reporter gene GFP (Nacalai Tesque, Kyoto, Japan), and the cell-type markers GSTpi (MBL Ltd, Tokio, Japan), CC1 (Calbiochem, San Diego, CA, USA), NG2 (Millipore, Burlington, MA, USA), PDGFRa (Rn’D systems, Minneapolis, MN), GFAP (Dako, Santa Clara, CA, USA), IBA1 (Wako, Osaka, Japan), and NeuN (Millipore, St. Louis, MO).

Techniques: Immunostaining, Recombinant, Staining

Journal: Journal of Nuclear Medicine

Article Title: PD-L1 Immuno-PET Reveals Systemic Effects of Localized Oncolytic Virotherapy in a Mouse Model of Head and Neck Cancer

doi: 10.2967/jnumed.125.270922

Figure Lengend Snippet: (A) Representative fluorescent images of MOC1 and MOC2 cells after 48 h of incubation with increasing titers of GFP-expressing RP1-15. GFP shown in gray scale. (B) MOC1 and MOC2 cell viability after 48 h with increasing RP1 titers in vitro, assessed via CellTiter-Glo assay. Significance determined using 1-way ANOVA with Dunnett test. RLU = relative light units.

Article Snippet: The MOC2( PD-L1 ) cell line was generated in our laboratory to overexpress PD-L1 by transducing MOC2 cells with a lentiviral vector carrying the murine CD274 gene tagged with a green fluorescent protein (GFP) reporter (MR 203953L2; OriGene Technologies).

Techniques: Incubation, Expressing, In Vitro, Glo Assay